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Synthetic Biology: Mechanistic Insights into Engineered Riboswitches - Beatrix Suess

iBiology Techniques
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https://www.ibiology.org/bioengineeri... Riboswitches are highly structured RNA elements that are located in the 5’ UTR of many bacterial mRNAs. Riboswitches bind ligands with high specificity. This causes a termination of transcription or the inhibition of translation initiation. Dr. Suess explains that it is possible to select for RNA aptamers that bind a specific ligand, such as tetracyline or neomycin, and engineer these into the 5’ region of a yeast mRNA allowing gene expression to be regulated by adding ligand. Suess discusses what makes an aptamer into a riboswitch. Speaker Biography: Beatrix Suess is a Professor at the Technical University, Darmstadt. She received her PhD from the University of Erlangen, Germany where she was also a post-doctoral fellow. Suess was also a research fellow at the University of Vienna, Austria and at Yale University, USA. Dr. Suess’s research focuses on the ability of RNA to operate as a regulator of activities within the cell. In particular, her work focuses on the use of riboswitches as regulatory devices in synthetic biology applications. ...more
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These chapters are auto-generated

Intro

0:00

RNA Can Form Alternative Structures

0:17

Riboswitches Use Alternative Structures

0:39

Advantages of Riboswitch Regulation

1:26

SELEX

2:28

RNA Aptamers as Genetic Control Device

4:55

The Tetracycline Aptamer

5:50

Aptamer-mediated Translational Inhibition

6:21

Control of Essential Genes in Yeast

6:40

Control of pre-mRNA Splicing - Yeast

7:27

Regulated RNA degradation...

9:50

Tetracycline-Dependent Ribozymes

10:42

RNA Synthetic Biology

13:52

What Turns an Aptamer into a Riboswitch?

14:58

The Neomycin Aptamer

16:50

The Free Aptamer is Largely Unfolded

17:55

Model for Neomycin-induced Conformational Changes

18:28

A 3-nt Element Keeps the Ground State Open

19:16

Ligand Binding by Conformational Capture

19:49

what about Inactive Neomycin Aptamers

20:21

Summary

20:36
Synthetic Biology: Mechanistic Insights into Engineered Riboswitches - Beatrix Suess
175Likes
11,667Views
2016Jan 5
https://www.ibiology.org/bioengineeri... Riboswitches are highly structured RNA elements that are located in the 5’ UTR of many bacterial mRNAs. Riboswitches bind ligands with high specificity. This causes a termination of transcription or the inhibition of translation initiation. Dr. Suess explains that it is possible to select for RNA aptamers that bind a specific ligand, such as tetracyline or neomycin, and engineer these into the 5’ region of a yeast mRNA allowing gene expression to be regulated by adding ligand. Suess discusses what makes an aptamer into a riboswitch. Speaker Biography: Beatrix Suess is a Professor at the Technical University, Darmstadt. She received her PhD from the University of Erlangen, Germany where she was also a post-doctoral fellow. Suess was also a research fellow at the University of Vienna, Austria and at Yale University, USA. Dr. Suess’s research focuses on the ability of RNA to operate as a regulator of activities within the cell. In particular, her work focuses on the use of riboswitches as regulatory devices in synthetic biology applications.

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iBiology Techniques

81.2K subscribers